All of the Seachem liquid supplements, medications, and water conditioners are designed to be used while the fish are still in the tank. The only time that Seachem recommends catching and removing your fish is when they are being moved to another tank, quarantine system, or dip solution. You might also want to remove the fish if you are making major alterations to aquarium decor or substrate.
That said, some fish are more sensitive to sudden changes in their water than others. If you are concerned that your fish may become stressed by the sudden addition of a product, it is recommended to start with 1/4 or 1/2 the standard dose and work up to the full dose over time.
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