The instructions are intended to bring the copper concentration up to the proper level over the course of 2 doses that are 48 hours apart. If you're dosing saltwater, the first dose should bring the copper concentration to 0.25 mg/L, and the second to 0.5 mg/L. The instructions are based on gallons (1 ml per 10.5 gallons). Be sure to estimate the correct amount of gallons in your aquarium for proper dosing. If your aquarium is a typical reef with a lot of rock, you have displaced some of the gallons of your aquarium.
Given that you have dosed your aquarium once with Cupramine™, I would test the level of copper to figure out what level you currently have. Ex., If you have dosed 3 ml and come out with .25 ppm of copper, you know dosing another 3 ml will raise the level another .25 ppm for a total of .50 ppm, which is the recommended concentration for this product. If the actual water volume of your aquarium is different from what you initially estimated, your copper concentration may be different. Keep in mind that this product is still effective as low as .20 ppm and generally safe for fish up to .80 ppm, but we recommend .50 ppm as the ideal level.
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